Salmonella enterica serotype typhi is a gram-negative bacterium that is responsible for typhoid fever and has been a burden on developing nations for generations. Typhoid fever is a bacterial infection due to a specific type of Salmonella that causes symptoms. They may vary from mild to severe, and usually begin 6 to 30 days after exposure. Often there is a gradual onset of a high fever over several days. A hospital based, prospective, cross-sectional study, was conducted in Microbiology laboratory-KMC hospital Ambedkar Circle from October 2012 to March 2014.The study included screening of 200 food handlers working in the mess of seven hostels, and in the canteens of three hospitals, for Salmonella carriage. Blood cultures were performed using the BacT/Alert system, Bio’Merieux. Isolates obtained from blood cultures were identified by the automated Vitek II system, Bio’Merieux. Studying the hygiene standards of the food handlers is necessary because, a good hygiene can reduce the transmission rate of various infections, especially enteric fever. RAPD is a rapid typing method based on random amplification of polymorphic DNA segments. Here, a short oligonucleotide of arbitrary sequence is used to prime DNA synthesis by accessing random segments of genomic DNA at low stringency to reveal polymorphism. Between 16 clinical isolates, 6 various Random Amplification of Polymorphic DNA (RAPD) were observed. Clinical S.Typhi isolates and isolates from healthy carriers are genetically different.
Meghna C, Vidyalakshmi K, Shrikala B