Background: Matrix-enclosed populations of bacteria known as Biofilms, stick to one another or to the different surfaces or interface. This definition comprises floccules, adherent populations and microbial clumps within the porous media having pore spaces. This ability of biofilm formation is a special property of bacteria. Multicellular communities existing in the natural environment known as biofilms are having unique architectural features by interstitial voids such as micro and macro colonies. Biofilms are basically an ordered aggregate of microorganisms living within their self-produced extracellular matrix and attach to the surfaces irreversibly but these aggregates are not easy to remove unless rinse quickly. In the attachment stage of biofilm to the surface, formation of (EPS) extracellular polymeric substances occurs. Phylogenetic history of different related biofilms can be find by using different computational techniques like tree viewer and quorum sensing. Biofilm formation is not a good thing in many ways so there must be some ways to stop the formation of biofilm so there are some anti-biofilm approaches and by using them we can stop the growth of biofilms. Some of these techniques include aptamers, enzyme treatment, nanoparticles, photo- dynamic therapy anti adhesion approaches etc. The development of anti-biofilm agents against different microbial targets and their subsequent application as adjuvants with antimicrobial agents seems to be more efficient.