Abstract

Evaluation of the Diagnostic Value of Direct Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry for Identifying Candida Species in Oral Candidiasis Patients

Oral Candidiasis is one of the most common oral mucosal infectious diseases in clinic. Accurate and rapid identification of yeasts is important to initiate appropriate treatment. In the first phase of the present study, 175 candida isolates were collected from non-stimulated whole saliva of 170 patients with oral candidiasis, and cultured on Sabouraud’s agar. Rapid Matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was compared to classical testing for identifying these Candida isolates. The accuracy was 94.3% and 84.0%, respectively, for C. albicans and non C. albicans isolates. In order to shortening the test time, 57 participants were enrolled in the second phase. Clinical candida isolates were collected from gargle specimens, cultured in liquid medium for less than 48 hours. The optimal accuracy of MALDI-TOF MS for gargle specimens was 76.1%, which were cultured in Sabouraud’s medium for 24 hours. MALDI-TOF MS is effective and rapid (12.7-24.7 h for turnaround time, 5.5 min for hands-on time per sample) for Candida spp. identification, and could be used as optional methods for rapid detection and diagnosis of oral candidiasis patients in clinic.


Author(s):

Pan W, Jingya F, Yifei Z, Xin L, Zhimin Y, Xiaobing G, Feng C, Xiaosong L



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